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Contact: Bella Smith
Company: Creative Biolabs
45-16 Ramsey Road
Shirley, New York 11967
United States
Phone: 1-631-619-7922
Fax: 1-631-207-8356
E-Mail: Send Inquiry
Date/Time:  1/4/18 12:09 GMT
 

antibody avidity

Affinity maturation is the process to improve antibody affinity for an antigen. In
vivo, natural affinity maturation by the immune system takes place by somatic
hypermutation and clonal selection. In vitro, in the laboratory affinity
maturation, can be obtained by mutation and selection.

Creative Biolabs has gained extensive experience in antibody affinity maturation.
We usually take scFv as the antibody format in affinity maturation. Also, a
monovalent display phagemid system is used to reduce the avidity effects during
antigen-binding screening. We also provide affinity maturation services for single
domain antibodies. Two methods, untargeted mutagenesis and oligonucleotide-
directed mutagenesis, are employed to construct random or defined sub-libraries to
introduce a large number of mutants of the original antibody. Antibody binders of
higher affinity are then selected by increasing the screening stringency. By
constructing a series of sub-libraries of a scFv/Fab antibody, our proprietary
protocol allows increase of the affinity of the scFv antibodies from 10 -9 to 10
-10. We have successfully obtained a scFv antibody that has an extremely high
affinity of 10 -12, whose binding to the antigen is essentially irreversible.

http://www.creative-biolabs.c , om/antibody-affinity-maturation.html
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