Ion Exchange Chromatography

In the downstream separation and purification process of biopharmaceuticals,
chromatography is one of the indispensable separation methods. Bio-Link
provides customers with 5 different chromatography solutions, including gel
filtration chromatography, ion exchange chromatography, affinity chromatography
, hydrophobic chromatography and multimodal chromatography. In addition, Bio-
Link also produces chromatography columns and prepacked columns from laboratory
to industrial grade. We focus on providing customers with high-quality
products, professional solutions and comprehensive after-sales services.

How To Select The Appropriate Chromatography Resin For My Own Process?
To select a suitable chromatography medium and chromatography mode, we need to
clarify the purpose of chromatography and the isoelectric point PI (charge
property) of the target product. The PI of the target product can be obtained
by calculating the target product sequence (theoretical isoelectric point), or
by capillary isoelectric focusing electrophoresis (CIEF) (actual isoelectric
point). Separation of substances based on the difference in charge between the
target product and impurities in a buffer with a specific pH value. According
to different chromatographic purposes, two modes can be selected for the ion
exchange chromatography process: (1) flow-through mode: the target product does
not bind to the packing material, and impurities are bound to the
chromatography packing material as much as possible; (2) combined elution mode
: The target product is combined with the filler, and then eluted with a
certain concentration of salt.

FAQs of Ion Exchange Chromatography

What is the principle of ion-exchange chromatography?

resin column chromatography is a chromatography method that separates
substances according to the difference in charge properties of different
biomolecules. The ion exchange chromatography medium is composed of
agarose/dextran/polymer matrix coupled with ligands with positive and negative
charges. The medium with negative charge is called cation exchange
chromatography medium, which can bind positively charged proteins; the medium
with negative charge is called anion exchange chromatography medium, which can
bind negatively charged proteins.

How many chromatography resin can Bio-Link produce per year?

The chromatographic medium manufacturing facility for Bio-Link is situated in
Lanzhou City, Gansu Province, and spans a total area of more than 7,000 square
meters. The factory is in charge of producing purification and cell culture
items such chromatography packing materials and microcarriers, with a total
investment of more than 100 million yuan and close to 100 workers. The base now
contains a production line for 500L and 1000L chromatography packaging, as well
as a well-equipped laboratory for quality testing (which has earned
ISO9001:2015 quality management system accreditation). The plant's 80,000
liters per year total capacity is envisaged.

As a professional lifescience company, BioLink is committed to continuously
providing customers with high-quality innovative products and solutions.

Minimum Order: 1 bags

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