Human Amyloid β Protein 40 ELISA kit

E01A0049 Human Amyloid β Protein 40 ELISA kit

Human Adenosine diphosphate ELISA kit is suitable for the detection of samples
from human species. Amyloid Beta ELISA Protein 40 can also be called Aβ40,
beta-amyloid 40.

Principle of the Assay

AΒ40 ELISA kit applies the quantitative sandwich enzyme immunoassay technique.
The microtiter plate has been pre-coated with a monoclonal antibody specific
for AΒ40. Standards or samples are then added to the microtiter plate wells
and AΒ40 if present, will bind to the antibody pre-coated wells. In order to
quantitatively determine the amount of AΒ40 present in the sample, a
standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal
antibody, specific for AΒ40 are added to each well to “sandwich” the AΒ40
immobilized on the plate. The microtiter plate undergoes incubation, and then
the wells are thoroughly washed to remove all unbound components. Next,
substrate solutions are added to each well. The enzyme (HRP) and substrate are
allowed to react over a short incubation period. Only those wells that contain
AΒ40 and enzyme-conjugated antibody will exhibit a change in color. The
enzyme-substrate reaction is terminated by addition of a sulphuric acid
solution and the color change is measured spectrophotometrically at a
wavelength of 450 nm. A standard curve is plotted relating the intensity of the
color (O.D.) to the concentration of standards. The AΒ40 concentration in each
sample is interpolated from this standard curve.

Quality Control on Human Amyloid β Protein 40 ELISA kit
Coefficient of Variance Intra Variation% ＜10%
Inter Variation% ＜12%
Recovery 92-105%
Linearity Diluent Ratio Range %
1:02 96-105
1:04 903-103
1:08 89-107
Specificity/Cross-reactivity No significant cross-reactivity or interference
between AΒ40 and analogues was observed.

Citations of Human Amyloid β Protein 40 ELISA kit
E01A0049 has been referenced in the below publications:

Therapeutic effect of probucol on mild cognitive impairment in type 2 diabetic
patients.

Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer's Disease-Like
Amyloid-β Pathology and Neuroinflammation in the Rat Hippocampus.

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production, and sale in one, our product's range includes Enzyme-Linked
Immunosorbent Assay (ELISA) kits and registered trademark "cgbio®" ELISA Kits,
proteins, antibodies, plasmids, and other reagents, such as His-tag Protein
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