Rat Epinephrine/Adrenaline ELISA kit

E02A0007 Rat Epinephrine/Adrenaline ELISA kit

Rat Adrenaline/Epinephrine ELISA kit is suitable for the detection of samples
from Rat species. Epinephrine/Adrenaline can also be called ADR, or EPI.

Specifications of Rat Adrenaline/Epinephrine ELISA kit
Product Information
Cat. No. E02A0007
Product Name Rat Epinephrine/Adrenaline ELISA kit
Species Rat
Product Size 48 Tests / 96 Tests
Concentration 100-2500pg/ml
Sensitivity 1.0 pg/mL
Principal Competitive ELISA
Sample Volume 100 ul
Sample Type Serum, plasma, cell culture supernatants, body fluid and tissue
homogenate
Assay Time 90 minutes
Platform Microplate Reader
Conjugate HRP
Detection Method Colorimetric
Storage 2-8°C

Principle of the Assay

EPI ELISA kit applies the competitive enzyme immunoassay technique utilizing an
anti-EPI antibody and an EPI-HRP conjugate. The assay sample and buffer are
incubated together with EPI-HRP conjugate in pre-coated plate for one hour.
After the incubation period, the wells are decanted and washed five times. The
wells are then incubated with a substrate for HRP enzyme. The product of the
enzyme-substrate reaction forms a blue colored complex. Finally, a stop
solution is added to stop the reaction, which will then turn the solution
yellow. The intensity of color is measured spectrophotometrically at 450nm in a
microplate reader. The intensity of the color is inversely proportional to the
EPI concentration since EPI from samples and EPI-HRP conjugate compete for the
anti-EPI antibody binding site. Since the number of sites is limited, as more
sites are occupied by EPI from the sample, fewer sites are left to bind EPI-HRP
conjugate. A standard curve is plotted relating the intensity of the color
(O.D.) to the concentration of standards. The EPI concentration in each sample
is interpolated from this standard curve.

Citations of Rat Epinephrine/Adrenaline ELISA kit
E02A0007 has been referenced in the below publications:

Role of plateau hypoxia hypothermia complex damage to the vascular endothelial
cells and the protective effect.

The mechanism research of neurons injury in the basolateral of amygdala induced
by stress.

If you want to know more kinds of human igg elisa kits, please visit our
website.

As one elisa kit manufacturer, we are committed to providing the kits with
simple and easy operations but accurate and reliable quality to the
researchers, and we have won a good reputation from customers all around the
world. Many published papers have cited the usage of BlueGene Biotech ELISA
kits. In addition, we provide a 100% Risk-Free Performance Guarantee,
professional technical service, and timely assistance for all customers. Our
strict and sound quality control is a key part to sustainably supply our
products for the international market for more than 12 years. More new products
with good quality are coming soon...

Minimum Order: 1 pieces

Click to Enlarge