Human Asymmetric Dimethylaoyoinine ELISA kit

E01A0035 Human Asymmetric Dimethylaoyoinine ELISA kit

Human Asymmetric Dimethylaoyoinine ADMA ELISA kit is suitable for the detection
of samples from human species. Asymmetric Dimethylaoyoinine can also be called
N-Dimethylarginine: NG, NG-Dimethylarginine dihydrochloride, Asymmetric
Dimethylarginine, ADMA.

Specifications of Human Asymmetric Dimethylaoyoinine ELISA kit
Principle of the Assay

ADMA ELISA kit applies the quantitative sandwich enzyme immunoassay technique.
The microtiter plate has been pre-coated with a monoclonal antibody specific
for ADMA. Standards or samples are then added to the microtiter plate wells and
ADMA if present, will bind to the antibody pre-coated wells. In order to
quantitatively determine the amount of ADMA present in the sample, a
standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal
antibody, specific for ADMA are added to each well to “sandwich” the ADMA
immobilized on the plate. The microtiter plate undergoes incubation, and then
the wells are thoroughly washed to remove all unbound components. Next,
substrate solutions are added to each well. The enzyme (HRP) and substrate are
allowed to react over a short incubation period. Only those wells that contain
ADMA and enzyme-conjugated antibody will exhibit a change in color. The enzyme-
substrate reaction is terminated by addition of a sulphuric acid solution and
the color change is measured spectrophotometrically at a wavelength of 450 nm.
A standard curve is plotted relating the intensity of the color (O.D.) to the
concentration of standards. The ADMA concentration in each sample is
interpolated from this standard curve.

Quality Control on Human Asymmetric Dimethylaoyoinine ELISA kit
1:08 93-109
Specificity/Cross-reactivity No significant cross-reactivity or interference
between ADMA and analogues was observed.

Citations of Human Asymmetric Dimethylaoyoinine Bluegene ELISA kit
E01A0035 has been referenced in the below publications:

Dickkopf1 destabilizes atherosclerotic plaques and promotes plaque formation by
inducing apoptosis of endothelial cells through activation of ER stress.

Brain Volumetrics, Regional Cortical Thickness and Radiographic Findings in
Adults with Cyanotic Congenital Heart Disease.

Effect of super-flux polyethersulfone dialysis membrane on the removal of adma
asymmetric dimethylarginine: a pilot clinical study.

The Effect and Mechanism of Asymmetric Dimethylarginine Regulating
Trophoblastic Autophagy on Fetal Growth Restriction.

The role and mechanism of asymmetric dimethylarginine in fetal growth
restriction via interference with endothelial function and angiogenesis.

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